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Water Science & Technology Vol 66 No 3 pp 594–602 © IWA Publishing 2012 doi:10.2166/wst.2012.217
Efficient biodegradation of naphthalene by a newly characterized indigenous Achromobacter sp. FBHYA2 isolated from Tehran Oil Refinery Complex
Sima Farjadfard, Seyyed Mehdi Borghei, Amir Hessam Hassani, Bagher Yakhchali, Mehdi Ardjmand and Majid Zeinali
Department of Environmental Engineering, Graduate School of the Environment and Energy, Science and Research Branch, Islamic Azad University, Simon Bolivar Boulevard, Ashrafi Esfehani Highway, Poonak Square, Tehran, Iran Email: simafarjadfard@yahoo.com
Biochemical and Bioenvironmental Research Center, Department of Chemical and Petroleum Engineering, Sharif University of Technology, Azadi Street, Tehran, Iran
National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh Km 15, Tehran-Karaj Highway, Tehran, Iran
Department of Chemical Engineering, Tehran South Branch, Islamic Azad University, Ahang Street, Nabard Street, Piroozi Avenue, Tehran, Iran
Biotechnology Research Center, Research Institute of Petroleum Industry (RIPI), West Boulevard of Azadi Sport Complex, Tehran, Iran
ABSTRACT
A bacterial strain, FBHYA2, capable of degrading naphthalene, was isolated from the American Petroleum Institute (API) separator of the Tehran Oil Refinery Complex (TORC). Strain FBHYA2 was identified as Achromobacter sp. based on physiological and biochemical characteristics and also phylogenetic similarity of 16S rRNA gene sequence. The optimal growth conditions for strain FBHYA2 were pH 6.0, 30 °C and 1.0% NaCl. Strain FBHYA2 can utilize naphthalene as the sole source of carbon and energy and was able to degrade naphthalene aerobically very fast, 48 h for 96% removal at 500 mg/L concentration. The physiological response of Achromobacter sp., FBHYA2 to several hydrophobic chemicals (aliphatic and aromatic hydrocarbons) was also investigated. No biosurfactant was detected during bacterial growth on any aliphatic/aromatic hydrocarbons. The results of hydrophobicity measurements showed no significant difference between naphthalene- and LB-grown cells. The capability of the strain FBHYA2 to degrade naphthalene completely and rapidly without the need to secrete biosurfactant may make it an ideal candidate to remediate polycyclic aromatic hydrocarbon (PAH)-contaminated sites.
Keywords: Achromobacter sp.; biodegradation; biosurfactant; naphthalene; surface hydrophobicity
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