
Water Science & Technology Vol 66 No 2 pp 385393 © IWA Publishing 2012 doi:10.2166/wst.2012.198
Ecofriendly approach for detection of phenols in water using laccase from different fungi
Mazaahir Kidwai, Arti Jain, Abha Sharma and Ramesh Chander Kuhad
Department of Chemistry, Green Research Laboratory, University of Delhi, North Campus, New Delhi 110007, India E-mail: kidwai.chemistry@gmail.com
Department of Microbiology, Lignocellulose Biotechnology Laboratory, University of Delhi, South Campus, New Delhi 110021, India
Presently Vice Chancellor Of Jiwaji University, Gwalior, M.P., India
ABSTRACT
Laccase-initiated oxidative coupling reactions of phenol and its derivatives with 4-aminoantipyrene using air as an oxidant has been investigated. The oxidation reaction of phenols and 4-aminoantipyrene is getting a lot of attention due to environmental concerns. Oxidation of simple phenol and 4-aminoantipyrene as a benchmark reaction enabled us to rank the relative oxidation ability of various laccases. Among the laccases tested, laccase from Pycnoporus cinnabarinus successfully yielded 72% antipyrilquinoneimine dye. The present method can also be used to determine p-substituted phenols and chlorophenols. In this work, the influence of mediators on laccase activity has also been studied.
Keywords: antipyrilquinoneimine dyes; aqueous medium; basidiomycetous fungi; environmentally benign; phenols
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