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Water Science & Technology Vol 48 No 8 pp 239–246 © IWA Publishing 2003 Production of biopesticides using wastewater sludge as a raw material - effect of process parametersM.L. Tirado Montiel*, R.D. Tyagi**, J.R. Valero*** and R.Y. Surampalli*****I.N.R.S. - ETE, Université du Québec, 2700 rue Einstein, Ste-Foy, Québec, Canada ABSTRACT Production of Bacillus thuringiensis (Bt) based biopesticide was studied using hydrolysed or raw wastewater sludge as a raw material. The sludge hydrolysis was carried out at different pH (2, 4 and 6). The sludge after hydrolysis was sterilised at 121°C for 30 minutes. The effect of temperature, pH and agitation speed on growth and toxin production was also investigated. The pH in the shake flask was controlled by adding 0.1 M TRIS buffer. The progress of the biopesticide production process was monitored by measuring viable cell count (VC), spore count (SC) and entomotoxicity (Tx). The entomotoxicity was measured against spruce budworm. Control of pH substantially increased the entomotoxicity of the final product. Increased agitation speed resulted in higher VC, SC and Tx values. eProduct: Buy this article for £24.00 (IWA MEMBER PRICE: £18.00) All prices include VAT. For customers where VAT should not be applied, the VAT amount will be removed upon payment |
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