| IWA Publishing Journals | | | Subscriptions | | | Authors | | | Users | | | Librarians | | | FAQs |
 |
Water Science & Technology Vol 48 No 8 pp 135–141 © IWA Publishing 2003 The microbial community analysis of a 5-stage BNR process with step feed systemH.W. Lee*, S.Y. Lee**, J.O. Lee***, H.G. Kim****, J.B. Park*****, E. Choi****** and Y.K. Park********Graduate School of Biotechnology, Korea University, 1 Anam-dong, Seoul 136-701, Korea ABSTRACT The microbial communities of 5-stage BNR activated sludge samples were analyzed using fluorescence in-situ hybridization (FISH) and 16S rDNA characterization. The total cell numbers of each reactor were from 2.36  109 cells/ml to 2.83 109 cells/ml. From 56.5% to 62.0% of total DAPI cell counts were hybridized to the most bacterial specific probe EUB 338. Among them, b-proteobacteria were most dominant in each tank. The number of phosphate accumulating organisms (PAOs) was almost 50% of the total cell number in anoxic-1 tank, and these results indicate that this process has a high content of denitrifying phosphorus accumulating organisms (dPAOs). In contrast with FISH, 16S rDNA analysis showed that dominant groups were the Cytophaga-Flavobacterium group and high G+C% gram-positive bacteria, which were determined as PAOs in anoxic-1 tank. The beta subclass Proteobacteria did not accumulate a large amount of polyphosphate. The overall results indicate that high G+C% gram-positive bacteria and the Cytophaga-Flavobacterium group might play a key role as dPAOs in this process.
eProduct: Buy this article for £24.00 (IWA MEMBER PRICE: £18.00) All prices include VAT. For customers where VAT should not be applied, the VAT amount will be removed upon payment |
|
 |
