Water Science and Technology Vol 35 No 11-12 pp 415–418 © IWA Publishing 1997
Rapid detection of coliform bacteria and influence of non-target bacteria
L. Fiksdal*, I. Tryland** and H. Nelis**
*Department of Hydraulic and Environmental Engineering, Norwegian University of Science and Technology 7034 Trondheim Norway
**Laboratory of Pharmaceutical Microbiology, University of Gent 9000 Gent Belgium
ABSTRACT
Enzymatic hydrolysis of fluorogenic substrates (4-methylumbelliferyl- -D-galactoside, 4-methylumbelliferyl--D-glucuronide) has been used for rapid (25min) detection of indicators of faecal water pollution, i.e. coliform and thermotolerant coliform bacteria. In the present work, enzymatic activities and different groups of bacteria (i.e. thermotolerant coliforms, coliforms, -galactosidase positive and -glucuronidase positive bacteria) from sewage effluent and polluted river water have been investigated. Ratios of the order of 1:10 between coliforms and -galactosidase positive bacteria were demonstrated. The contribution from -galactosidase positive non-coliform bacteria to the measured enzyme activity should not, therefore, be neglected. Numbers of -glucuronidase positive non-coliforms indicate interference of this group in the rapid assay.
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