Water Science & Technology: Water Supply Vol 1 No 2 pp 107–114 © IWA Publishing 2001
Removal of tetrachloroethylene in an anaerobic fixed-bed reactor
immobilized with Clostridium bifermentans DPH-1
YC Chang*, K Asanuma**, M Hatsu*** and K Takamizawa****
*
Department of Bioprocessing, Faculty of Agriculture, Gifu University,
1-1 Yanagido, Gifu 501-1193, Japan E-mail: tak2003@cc.gifu-u.ac.jp
**
Department of Bioprocessing, Faculty of Agriculture, Gifu University,
1-1 Yanagido, Gifu 501-1193, Japan E-mail: tak2003@cc.gifu-u.ac.jp
***
Department of Bioprocessing, Faculty of Agriculture, Gifu University,
1-1 Yanagido, Gifu 501-1193, Japan E-mail: tak2003@cc.gifu-u.ac.jp
****
Department of Bioprocessing, Faculty of Agriculture, Gifu University,
1-1 Yanagido, Gifu 501-1193, Japan E-mail: tak2003@cc.gifu-u.ac.jp
ABSTRACT
An upflow fixed-bed reactor was evaluated for the removal of
tetrachloroethylene (PCE) by a strictly anaerobic, PCE dechlorinating
bacterium, Clostridium bifermentans DPH-1 immobilized on sterile ceramic
beads. The ceramic beads provide a large surface area for development
of cell mass in the column. For kinetic experiments, the conventional
first-order reaction kinetics was applied to evaluate the batch and
continuous immobilized reactors. Velocity constants (KB and
KC) under the batch culture and continuous culture were
determined to be 1.9  10–2
(mg protein–1h–1) and
1.4 10–2
(mg protein–1h–1), respectively.
Maximum specific degradation rate of PCE was calculated as 0.16
(10–3  mol
mg protein–1h–1 from average influent
concentration of PCE (12 M) in the bioreactor. Simultaneously
maximum volumetric degradation rate was also computed as
10.2 mol l–1h–1. The volumetric
degradation rate was relatively higher than those of other reactors. In
order to maintain the efficiency of PCE dechlorination, 20 h or more HRT
in the reactor system was required.
Full article (PDF Format)
PAY-PER-VIEW: Buy this article for £20.00 (IWA MEMBER PRICE: £15.00)
|
