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Journal of Water Supply: Research and Technology—AQUA Vol 56 No 3 pp 191–202 © IWA Publishing 2007 doi:10.2166/aqua.2007.103
Optimization of the detection of the spores of aerobic spore-forming bacteria (ASFB) in environmental conditions
Clément Cartier, Benoit Barbeau, Marie Claude Besner, Pierre Payment and Michèle Prévost
City of Montréal, Infrastructure, Transport and Environmental services, Water Systems Strategic Planning, Montréal, (Québec) Canada H3A 3P1,
NSERC Industrial Chair on Drinking Water, École Polytechnique de Montreal, Department of Civil, Geologic and Mining Engineering, P.O. Box 6079, Succ. Centre Ville, H3C 3A7, Montreal, (Quebec) Canada
École Polytechnique de Montreal, Department of Civil, Geologic and Mining Engineering, P.O. Box 6079, Succ. Centre Ville, Montreal, (Quebec) Canada H3C 3A7,
INRS – Institut Armand-Frappier, 531 boul. des Prairies, Laval, (Québec) Canada H7V 1B7,
NSERC Industrial Chair on Drinking Water, École Polytechnique de Montreal, Department of Civil, Geologic and Mining Engineering, P.O. Box 6079, Succ. Centre Ville, Montreal, (Quebec) Canada H3C 3A7, Tel.: 1(514) 340 4711 (5924)Fax: 1 (514) 340 5918michele.prevost@polymtl.ca
ABSTRACT
The measurement of spores of aerobic spore-forming bacteria (ASFB) is becoming a widely accepted method for validating the effectiveness of treatments applied in drinking water treatment plants. These bacteria have been proposed as indicators of soil intrusion in distribution systems. There are, nevertheless, some limitations to the measurement of ASFB spores by membrane filtration with respect to the rate of recovery of spores present in distributed water. This is mainly due to the low concentrations of these spores. Two factors may decrease spore recovery: the presence of a cake on the filter and the aggregation of spores. For distributed water, our data suggest limiting the volume of water filtered (V in ml) as a function of its turbidity, according to the relation: V = 500/turbidity. The addition of a surfactant, Tween 80®, can increase the recovery of spores very significantly (1000 X). The experimental results show that a triple manual inversion will ensure a significantly higher rate of recovery.
Keywords: aerobic spores; ASFB; distribution system; drinking water; indicator; intrusion; spores
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