Journal of Water Reuse and Desalination Vol 1 No 1 pp 18–26 © IWA Publishing 2011 doi:10.2166/wrd.2011.030
Decolorization of industrial azo dye in an anoxic reactor by PUF immobilized Pseudomonas oleovorans
E. Silveira, P. P. Marques, A. C. Macedo, P. G. Mazzola, A. L. F. Porto and E. B. Tambourgi
School of Chemical Engineering, State University of Campinas, Unicamp, P.O. Box 6066, 13083-970 Campinas, SP, Brazil E-mail: silveiraedgar@gmail.com
Faculty of Medical Sciences, State University of Campinas, Unicamp, P.O. Box 6111, 13083-970 Campinas, SP, Brazil
DMFA/Cenapesq, Federal Rural University of Pernambuco, UFRPE, Dois Irmãos, 52171-900 Recife, PE, Brazil
ABSTRACT
This work reported the full degradation of an azo dye in a synthetic effluent by Pseudomonas oleovorans immobilized in polyurethane foam (PUF). For each fed-batch experiment, a screw-top vessel containing 160 mL of nutrient broth was inoculated with 0.16 g L-1 of fresh culture, incubated at 32 °C and supplemented with 50 mg L-1 of dye every 24 hours. Afterwards, the P. oleovorans were immobilized in PUF and inoculated in an anoxic reactor. The results showed that at fed-batch conditions, P. oleovorans was capable of removing 50 mg of dye in 192 hours. However, when the decolorization was performed in an anoxic reactor, it was capable of fully degrading 25 mg of dye in only 24 hours.
Keywords: anoxic reactor; azo dye; immobilization; polyurethane foam; Pseudomonas
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